human ins  (Elabscience Biotechnology)

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) experiments were conducted using the Mouse/Rat ACTH ELISA Kit (Abcam, #ab263880) to detect ACTH in AtT20 cell experiments, and the Human Insulin (INS) ELISA Kit (Elabscience, #E-EL-H2665) to detect insulin secretion in NT-3 cells as reported ( ).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of the test and control SRLs on insulin secretion of NT-3 cells in the 2D culture model. (A, B) NT-3 cells were seeded at a density of 20, 000 cells/well and then were treated with the indicated concentrations (i.e. 5nM, 10nM, 50nM) of (A) the control SRL octreotide or with (B) the test peptides. After 5 days, supernatants were collected, and insulin ELISA was performed using the kit from Elabscience. The experiment was repeated twice with similar results. Data shown are the average of 2 technical replicates ± standard deviation (SD). NT, untreated. (C) The decrease in insulin secretion upon treatment with the test peptides is here compared with that obtained with octreotide. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-C) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) experiments were conducted using the Mouse/Rat ACTH ELISA Kit (Abcam, #ab263880) to detect ACTH in AtT20 cell experiments, and the Human Insulin (INS) ELISA Kit (Elabscience, #E-EL-H2665) to detect insulin secretion in NT-3 cells as reported ( ).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of octreotide and SMTR-002 on insulin secretion and viability of NT-3 cells in the 3D culture model. (A, B) NT-3 cells were seeded at a density of 20, 000 cell/well in ULA plates and allowed to form spheres (5 days). Then they were treated with (A) octreotide or (B) the lead peptide (=SMTR-002) at the indicated concentrations (5nM, 10nM, 50nM). After 5 days, supernatants were collected, and insulin concentration quantified using the Human Insulin (INS) ELISA Kit (Elabscience). Data shown are the average of 3 technical replicates ± standard deviation (SD). NT, untreated. (C, D) In cultures parallel to panels A and B, Cell viability was carried out with CellTiter-Glo 3D assay. The experiment was repeated 3 times with similar results. Data shown are the average of 3 technical replicates ± SD. NT, untreated. Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) experiments were conducted using the Mouse/Rat ACTH ELISA Kit (Abcam, #ab263880) to detect ACTH in AtT20 cell experiments, and the Human Insulin (INS) ELISA Kit (Elabscience, #E-EL-H2665) to detect insulin secretion in NT-3 cells as reported ( ).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation